Optimization of Initial Cell Density and Usage Efficiency of Seed Culture Medium During Lipid Production from Distillery Wastewater by Rhodosporidium toruloides

Authors

  • A. Jiayin Ling Author
  • B. Saiwa Nip Author
  • C. Renata Alves de Toledo Author
  • D. Hojae Shim Author

DOI:

https://doi.org/10.24084/repqj12.259

Keywords:

Initial cell density, lipid, non-sterile distillery wastewater, oleaginous yeast, seed culture medium

Abstract

For the environmentally sustainable development, the recovery of resource or energy from wastes has attracted worldwide attention in recent years. The wastewater produced from distillery is more difficult to treat than the domestic wastewater due to the high organic loads. This study explores the possibility of developing a process, which could produce microbial lipid convertible to biodiesel by recovering nutrients from non-sterile wastewater while simultaneously removing part of organic matters through cultivating oleaginous microorganisms. Especially, it is intended to further optimize the initial cell density and the usage efficiency of seed culture medium. The lipid productivity by oleaginous yeast Rhodosporidium toruloides was studied using wastewater from rice wine distillery, at 30ºC under different initial cell densities (2 x 107, 1 x 107, 0.8 x 107, and 0.5 x 107 cells/mL) and incubation periods (2-5 days). Chemical oxygen demand (COD) removal efficiency, cell yield, and lipid yield were analyzed and the potential for bioenergy recovery from wastewater was assessed. The seed culture medium was found reusable for the second time without the addition of extra nutrient and possible for the third time with the addition of glucose.

Author Biographies

  • A. Jiayin Ling

    Department of Civil and Environmental Engineering, Faculty of Science and Technology,  
    University of Macau, Taipa, Macau SAR, China  

  • B. Saiwa Nip

    Department of Civil and Environmental Engineering, Faculty of Science and Technology,  
    University of Macau, Taipa, Macau SAR, China

  • C. Renata Alves de Toledo

    Department of Civil and Environmental Engineering, Faculty of Science and Technology,  
    University of Macau, Taipa, Macau SAR, China 

  • D. Hojae Shim

    Department of Civil and Environmental Engineering, Faculty of Science and Technology,  
    University of Macau, Taipa, Macau SAR, China 

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Published

2024-01-24

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Section

Articles